Speaker: Dr. Sébastien Rodrigue (Université de Sherbrooke)
Where: L-GE 110 | When: 1:15 pm "Whole-Genome Cloning and Genome Transplantation Using the Quasi-Minimal Bacterium Mesoplasma florum" Abstract: Previous work described the cloning of entire bacterial chromosomes in yeast and their transplantation into recipient bacteria. This technology allows the creation of synthetic genomes, potentially very different from natural versions, which can be brought to life by a transplantation procedure. However, much remains to be studied about the molecular mechanisms and limitations of this approach. We report the whole-genome cloning of Mesoplasma florum in Saccharomyces cerevisiae, and use this model to investigate the impact of introducing a bacterial chromosome in yeast cells. Our results indicate that in this context, the M. florum genome is subjected to very weak transcriptional activity. We next demonstrated that the M. florum genome cloned in yeast could be transplanted into Mycoplasma capricolum. However, we obtained relatively low efficiencies compared to more phylogenetically related bacterial genomes that have been transplanted in the same recipient strain. We also sequenced the genomes of M. florum transplants and observed that small number of mutations appeared in every clone. Mutations also arose, although to a lesser extent, when the M. capricolum genome is inserted into a M. capricolum host. Taken together, our results offer a more detailed picture of the events that can affect bacterial genomes subjected to cloning and transplantation. Photo By: Michael Chung Comments are closed.
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